Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | concentration of 4.0 mM: activity reaches 171% | Geobacillus stearothermophilus | |
D-galactosamine | concentration of 100 mM: activity reaches 140% | Geobacillus stearothermophilus | |
D-glucosamine | concentration of 30 mM: activity reaches 140% | Geobacillus stearothermophilus | |
Fe2+ | concentration of 0.6 mM: activity reaches 204% | Geobacillus stearothermophilus | |
Zn2+ | concentration of 0.4 mM: activity reaches 142% | Geobacillus stearothermophilus |
General Stability | Organism |
---|---|
at 70°C for 10 min: most stable in potassium phosphate buffer, more stable under alkaline than acidic conditions | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chitobiose | concentration of 10 mM causes 25% inhibition | Geobacillus stearothermophilus | |
D-mannose | concentration of 10 mM causes 33% inhibition | Geobacillus stearothermophilus | |
fructose | concentration of 10 mM causes 38% inhibition | Geobacillus stearothermophilus | |
galactose | concentration of 10 mM causes 15% inhibition | Geobacillus stearothermophilus | |
glucose | concentration of 10 mM causes 30% inhibition | Geobacillus stearothermophilus | |
Hg2+ | - |
Geobacillus stearothermophilus | |
lactose | concentration of 10 mM causes 25% inhibition | Geobacillus stearothermophilus | |
Mn2+ | slight inhibition | Geobacillus stearothermophilus | |
N-acetyl-D-galactosamine | concentration of 10 mM causes 28% inhibition | Geobacillus stearothermophilus | |
N-acetyl-D-glucosamine | competitive inhibitor; concentration of 10 mM causes 80% inhibition | Geobacillus stearothermophilus | |
sucrose | concentration of 10 mM causes 9% inhibition | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
4-nitrophenyl N,N'-diacetylchitobioside | - |
Geobacillus stearothermophilus | |
0.17 | - |
4-nitrophenyl N,N',N''-triacetylchitotrioside | - |
Geobacillus stearothermophilus | |
0.28 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Geobacillus stearothermophilus | |
0.57 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Geobacillus stearothermophilus | |
0.59 | - |
p-nitrophenyl-thio-beta-N-acetyl-D-glucosaminide | - |
Geobacillus stearothermophilus | |
4.1 | - |
(N-acetyl-beta-D-glucosamine)2 | - |
Geobacillus stearothermophilus | |
8 | - |
(N-acetyl-beta-D-glucosamine)3 | - |
Geobacillus stearothermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Geobacillus stearothermophilus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
75000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Geobacillus stearothermophilus | - |
N-acetyl-D-hexosamines | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | - |
Geobacillus stearothermophilus |
dimethyl sulfoxide | 80%, 18.0% of activity remains | Geobacillus stearothermophilus |
dimethylformamide | 80%, 13.9% of activity remains | Geobacillus stearothermophilus |
dioxane | - |
Geobacillus stearothermophilus |
Ethanol | - |
Geobacillus stearothermophilus |
guanidine-HCl | 4 M at 37°C for 30 min, 54% of activity remains | Geobacillus stearothermophilus |
n-Butanol | - |
Geobacillus stearothermophilus |
n-propanol | - |
Geobacillus stearothermophilus |
Pyridine | - |
Geobacillus stearothermophilus |
sodium dodecyl sulfate | 0.1% and 1% SDS decrease activity to 66% and 26% | Geobacillus stearothermophilus |
urea | 8 M at 37°C for 30 min, 91% of activity remains | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
CH-4 | - |
Geobacillus stearothermophilus CH-4 | - |
CH-4 | - |
Purification (Comment) | Organism |
---|---|
387fold to homogeneity | Geobacillus stearothermophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Geobacillus stearothermophilus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.7 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(N-acetyl-beta-D-glucosamine)2 + H2O | - |
Geobacillus stearothermophilus | 2 N-acetyl-beta-D-glucosamine | - |
? | |
(N-acetyl-beta-D-glucosamine)3 + H2O | - |
Geobacillus stearothermophilus | (N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N,N',N''-triacetylchitotrioside + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + N,N',N''-triacetylchitotriose | - |
? | |
4-nitrophenyl N,N',N''-triacetylchitotrioside + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
4-nitrophenyl N,N'-diacetylchitobioside + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
4-nitrophenyl N,N'-diacetylchitobioside + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + N,N'-diacetylchitobiose | - |
? | |
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O | - |
Geobacillus stearothermophilus CH-4 | ? | - |
? | |
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | - |
Geobacillus stearothermophilus CH-4 | ? | - |
? | |
N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Geobacillus stearothermophilus | 2 N,N-diacetylchitobiose | - |
? | |
N,N',N''-triacetylchitotriose + H2O | - |
Geobacillus stearothermophilus | N-acetyl-D-glucosamine + N,N'-diacetylchitobiose | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Geobacillus stearothermophilus | deacetylated chitooligosaccharides + acetate | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Geobacillus stearothermophilus CH-4 | deacetylated chitooligosaccharides + acetate | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Geobacillus stearothermophilus | N-acetyl-D-hexosamines | - |
? | |
N-acetyllactosamine + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Geobacillus stearothermophilus | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Geobacillus stearothermophilus CH-4 | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Geobacillus stearothermophilus | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Geobacillus stearothermophilus CH-4 | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
p-nitrophenyl-thio-beta-N-acetyl-D-glucosaminide + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + thio-beta-N-acetyl-D-glucosaminide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 74000 | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for 10 min | Geobacillus stearothermophilus |
80 | - |
retains 28% of activity after 10 min | Geobacillus stearothermophilus |
90 | - |
complete inactivation after 10 min | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
stable | Geobacillus stearothermophilus |